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Metabolites were resolved across a 2.1 150 mm, 1.7 m Kinetex C18 column (Phenomenex) using a 5-min, reverse-phase gradient from a previously described method 19

Immunol Lett 115(1):2132

If the force was maintained for the required 3 seconds, a green tick indicated successful task performance during a single trial (see Supplementary Figure 3B)

(PubMed) Giustarini E, Pinchera A, Fierabracci P, et al

One- or two layer density gradient centrifugation alone may not be sufficiently discriminating to provide a pure preparation of Kupffer cells, but this technique can provide an important initial enrichment for these cells prior to the use of centrifugal elutriation, adherence of the Kupffer cells to a plastic surface