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Western blot analysis Whole cell lysates and lung homogenates were prepared with RIPA lysis buffer containing with a protease inhibitor cocktail (GenDEPOT

doi: 10.1016/s0006-8993(99)01825-9 [DOI] [PubMed] [Google Scholar] 98.Reber A, Budmiger B, Wenk M, Haefeli WE, Wolff T, Bein T, et al

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NNMTs role is to transfer a methyl group to nicotinamide (vitamin B3), creating 1-methylnicotinamide a reaction that consumes cellular methyl donors and depletes nicotinamide adenine dinucleotide (NAD) stores