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studied the radiation response of seven human cell lines (U-118-MG, U-251-MG, HTh-7, U-393-OS, U-622-CG, P7, and P31) by establishing survival curves as a function of varying radiation absorbed doses (110 Gy) (Table 2) [41]

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After a wash step, sections were incubated with anti-rabbit IgG horseradish peroxidase polymer (Cell Signaling Technology), diluted 1:1 with 0.1% vol/vol Tween-20 in PBS (wash buffer)

PMID 10650526