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Then, 6 Laemmli buffer (0.4 M SDS, 60 mM Tris, pH 6.8, 6.5 M glycerol, 0.6 M dithiothreitol, 0.9 mM bromophenol blue) was added, and samples were boiled for 5 min at 95 C

This, in turn, results in the activation of the unfolded protein response (UPR), the endoplasmic reticulum overload response, and the caspase-12-mediated apoptosis pathway

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