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Classification Congenital: Rare Includes: Enzymopathy autosomal recessive variants in the CYB5R3 gene Hemoglobinopathy (Hemoglobin M) autosomal dominant variants in the globin genes Acquired: More common Mainly due to the exposure to substances that cause oxidation of Hb both directly or indirectly

Cells were seeded into a 384-well glass-bottom black plate (IBL, P384-1.5H-N) and left to adhere overnight

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This advanced delivery system creates microscopic spheres that protect glutathione molecules from digestive degradation while facilitating direct cellular uptake