In this study, we have screened 78 different medicine herbal plants and identified that rhizome of Gastrodia elata is of great potency for tyrosinase inhibition
Protein extracts were separated on 8% SDS-polyacrylamide or 412% bistris protein gel (Invitrogen), transferred on a nitrocellulose membrane, and stained with antibodies against ZO-1 (N1N2, GeneTex, dilution 1:3000)
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It modulates specific protein phosphorylation process and intracellular Ca++ concentration through one sperm-specific Ca++ permeable channel (CatSper) in the plasma membrane of the flagellar principal piece, hence it may be beneficial to sperm motility [75,76,77,78,79]
For histopathology, fixed liver tissues were dehydrated, embedded in paraffin, and sectioned at 4 m for hematoxylin-eosin (H&E) staining