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Immunoblotting Cells were washed with PBS at the end of treatments if applicable, and then lysed with an appropriate volume of 2X Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) with 5% -mercaptoethanol (Bio-Rad Laboratories)

Biological Activity

Autophagy inhibition in those tumors affects multiple aspects of cancer metabolism such as lipid metabolism (233), amino acid recycling (234) and nucleotide homeostasis (235), making it a promising therapeutic strategy for RAS-driven cancers

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