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Low molecular mass thiols, disulfides and protein mixed disulfides in rat tissues: Influence of sample manipulation, oxidative stress and ageing

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Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

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To report continuous sale or distribution of the above unregistered food supplement, utilize our online reporting facility, eReport , at www.fda.gov.ph/ereport , or e-mail us via [email protected] , or call us at the Center for Food Regulation and Research (CFRR) hotline (02) 857-1900 loc