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Regulatory Pathways and Transformation in GSH Metabolism GSH metabolism also has specialized branches and is precisely regulated by multiple regulatory mechanisms to adapt to the needs of different tissues, organelles and cell states

After washing of trapping column with 0.1% FA, the peptides were eluted (flow rate300 nl/min) from the trapping column onto an analytical column (Aurora C18, 75 m ID, 250 mm long, 1.6 m particles, Ion Opticks) by 101 min long linear gradient (2-37% of mobile phase B

Under oxidative stress, accumulation of ROS can activate pathways such as the JNK and the p53, further inducing autophagy, which helps clear damaged organelles, reduces oxidative damage, and protects cell survival [256]

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