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Cells were loaded with MitoTracker Red dye (Excitation/Emission: 579/599 nm) at 100 nM final concentration (37 C for 15 min) mounted in Live Cell Imaging Solution (Thermo Fisher), and viewed under a laser confocal microscope (LSM 510

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We recorded the number of rats that maintained the initial position of firmly standing on the surface with posterior legs, with only limited movements around, as well as the number of those demonstrating no freezing behavior, quick exploration, and escaping from the elevated surface

10.1007/s00198-008-0764-2 350