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Oocytes were microinjected with two types of mRNA including MAP4-eGFP, to label microtubules, and H2B-mCherry, to label chromatin, followed by incubation for one hour in M2 medium

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Although the mechanisms of acquired resistance in situ are still poorly understood, studies in vitro have shown that cells selected for resistance to one drug often exhibit cross-resistance to other seemingly unrelated agents, suggesting a somewhat generalised mechanism of resistance

1c, Supplementary Figs 1 and 2)

2b, d) but appeared mostly in the cytosolic fraction upon cell fractionation (Fig