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Thus, the optimal experimental conditions for this strategy were determined to be 30 , using PBS buffer at pH 6.5 for a reaction time of 20 min

For biochemical assays, each 0.5 g sample of leaf material was homogenized in 3 mL 25 mM HEPES buffer (pH 7.8) containing 0.2 mM EDTA and 2% (w/v) polyvinylpyrrolidon