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Cells are grown for 48-72 hours to achieve 80-90% confluency before treatment

Cell Death Differ (2018) 25(8):145772

Although BSO is the most utilized treatment to achieve GSH depletion, other strategies such as inhibition of cysteine/glutamate transport showed a synergistic effect in combination with gemcitabine for inhibiting proliferation of pancreatic cancer cell lines[50]