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After transfer, membranes were blocked in 1% bovine serum albumin in PBS with 0.1% Tween-20 and probed with anti-PARP (NB100-56599, Novus Biologicals, Littleton, CO), anti-Glutathione (AB19534, Abcam, Cambridge, MA), anti-phospho-IB- (9246), anti-IB- (9242), anti-phospho-IKK- (2594), anti-IKK- (2370), or anti-p65 (8242) (Cell Signaling Technology, Danvers, MA) antibodies
Cycling advantages include reduced annual costs through intermittent purchasing (30-40% savings vs continuous use), potentially improved responsiveness when restarting after breaks avoiding adaptation, psychological freedom from continuous dependency, and accommodation of life changes, travel, or budget fluctuations
Kopetz S
Cytotoxicity and DNA damage properties of tert-butylhydroquinone (TBHQ) food additive
Kuriakose J, Bharat Bhushan N, et al
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