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We then conducted Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and Gene Ontology (GO) analyses to explore the biological functions of the upregulated and downregulated DEGs (Fig

The sorted cells were washed twice with 50 mL phosphate-buffered saline to remove the FreSHtracer and then further cultivated in culture medium for 24 h prior to in vitro or in vivo experiments, as described below

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