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The formation of gamma-glutamylcysteine is the rate-limiting reaction in GSH synthesis and is feedback inhibited by GSH itself, a mechanism responsible for the regulation of cellular GSH concentration

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Supplementation with 300-600 mg/day has been shown to increase intracellular glutathione levels by 30-70% in cell culture and animal studies, with more modest increases in human trials (Suh et al., Proc Natl Acad Sci, 2004) [25]

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