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Discussion The case of the 34-year-old male patient who presented with severe left leg pain, swelling, and tenderness is a typical example of the complications that can arise from intravenous drug abuse (IVDA)

In MAFLD, adipose tissue hypertrophy alters plasma exosomal miRNA distribution, disrupting glucose-lipid homeostasis [62]

Reactions without the phi 29 polymerase enzyme were included as a negative control

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