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HIV-1 tat impairment of mitochondrial respiration via complexes I and II can be ameliorated by allopregnanolone in opioid-exposed or opioid-nave cells and mice

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The membrane was incubated for 1 h in blocking buffer composed of Tween-Tris buffered saline (TTBS, 100 mM Tris, pH 7.5, 150 mM NaCl, 0.2% Tween 20) and 5% nonfat dry milk at 37C