For western blot-based competitive ABPP with RSL3-alkyne or SCLA2a samples were washed in 500-l solutions of: 1x 500 l 1% SDS in 50 mM HEPES and 2x 500 l 0.2% SDS in 50 mM HEPES
Early pharmacology work described it as a selective growth-hormone secretagogue, with GH release and little ACTH or cortisol signal in the foundational studies
Research also suggests it may help regulate sebum and keratin production
1 H NMR (300 MHz, DMSO) 8.26 (d, J = 7.6 Hz, 1H), 7.45 7.34 (m, 9H), 7.25 (d, J = 1.4 Hz, 1H), 7.13 7.03 (m, 6H), 7.03 6.95 (m, 1H), 6.70 6.61 (m, 1H), 4.49 (q, J = 6.9 Hz, 1H), 3.54 (s, 3H), 3.52 (d, J = 5.9 Hz, 1H), 2.82 (dd, J = 6.7, 3.4 Hz, 2H), 1.35 (s, 9H)
Underarm skin thats darker than the rest of the skin can keep some people from dressing in sleeveless shirts, wearing bathing suits in public, or participating in sports