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After 2 days of expression, cells were harvested in ice-cold PBS and subsequently lysed in AP-MS buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 10% Glycerol, 0.5 mM EDTA, 0.5% Nonidet P40) supplemented with protease inhibitor cocktail (Roche, Rotkreuz, Switzerland) by sonification, followed by centrifugation (20,000 g for 10 min

Supplied at 98% HPLC purity and batch-characterised for identity and purity, the single-vial format gives researchers a reliable, reproducible reference

Documented practice is to remove the vial, draw the dose, and return it to refrigeration rather than leaving it on the counter during prep

Preclinical data suggests it may be 10 million times more potent than BDNF at promoting synaptogenesis

Avoid in pregnant or breastfeeding individuals due to a lack of appropriate data