Cleaned FASP eluates were evaporated completely in SpeedVac concentrator (Thermo Fisher Scientific) and transferred into LC-MS vial containing PEG (final concentration 0.001%, v/v) using 25 l of 50% acetonitrile (ACN) with 2.5% formic acid (FA) and 100 l of ACN
The N-terminal domain is primarily involved in binding glutathione, while the C-terminal domain contributes to the binding of various hydrophobic substrates
As many of the effects of glutamine and glutamate described in this review were concentration-dependent, future studies should address whether this differential intake contributes to the differential occurrence in immune-related conditions between formula-fed and breastfed children
et al., Synergistic induction of heme oxygenase-1 by the components of the antioxidant supplement Protandim, Free Radical Biology & Medicine, 46: 430-440 (2009), the entire disclosure of which is incorporated herein by reference
2.2 Search strategy A comprehensive literature search was performed across PubMed, Scopus, Web of Science, and Google Scholar for studies published between January 2000 and December 2024