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It is also worth noting that animal model studies have illustrated a mechanistic contribution of microbial TMA and TMAO generation to enhanced development of atherosclerosis, platelet reactivity and thrombosis, and both impairment in renal function and fibrosis, as well as adverse ventricular remodeling and heart failure (refs

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However, incorporating a control tube that contains the same biological sample but lacks glutathione substrate can help to identify and prevent the interfering effect of thiols, ensuring more reliable and accurate test results