High-pressure freezing, freeze substitution, and ultramicrotomy Sapphire discs with neurons were incubated in pre-mixed NbLumSyt1-ALFA-tag Nb:NbALFA-Gold complex in a conditioned medium or in conditioned medium containing only NbALFA-Gold for 45 min
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Specific concepts that we believe may be particularly noteworthy include: (1) Misfolding of innate CNS proteins, which then leads to their aggregation into potentially toxic oligomers or amyloids, leading to a toxic gain-of-function via numerous mechanisms including membrane disruption (Kandel et al., 2017
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