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doi: 10.1096/fj.10-177147 97 MapurangaJ.ZhangN.ZhangL.ChangJ.YangW

Pilot study of reloxaliase in patients with severe enteric hyperoxaluria and hyperoxalemia

Optional: ATP buffer: Tris HCl, pH 7.4, 50 mM, ATP 2 mM, MgSO4 10 mM Elution buffer: Tris base, pH 7.4, 125 mM, NaCl 150 mM, Triton X-100 0.1 % (v/v), Reduced glutathione 50 mM, DTT 1 mM Note: Optimal buffer conditions may vary depending on the protein of interest

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Weight loss accelerates during this window, and the phase 2 clinical trial data published in the New England Journal of Medicine confirms this dramatically