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Nature 1995

Ethics, reproducibility and methodological pitfalls Frequent pitfalls in cognitive peptide research No vehicle control in some old Russian studies: mandatory in any modern publication Non-randomisation : assigning animals to groups by order of arrival introduces systematic bias (weight, oestral cycle, cage stress) Non-blinded experimenter : behavioural tests are highly sensitive to expectations

To test the effect of Sp in paraquat-induced oxidative stress model, seven days old wild type flies, constitutively expressing mitochondrial (mt)-roGFP, were exposed to 10 mM paraquat for 24 h and then co-treated with 2.5 mM Sp for 24 h, prior to roGFP fluorescence estimation