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When SH-SY5Y cells were exposed to mercury, inducing a two-fold increase in tau hyperphosphorylation over a 9-hour period, pre-treatment with melatonin for 12 hours effectively reduced tau hyperphosphorylation due to this transition metal [116]

For CCR2 staining, decloaking was performed for 10 min at 110 C in TRIS/EDTA buffer with 0.05% Tween in a pressure cooker

Transthoracic echocardiography was performed one week post-operatively (day 8) to confirm post-MI pathology

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