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For example, glucose and amino acid availability affect granulosa cell support and follicular fluid composition, and when it is disrupted, meiotic competence is impaired

Journal of Orthopaedic Research , 24(7), 1421-1432.

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Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders MDCK cells were grown to 100% confluency on the coverslips in six-well plates and washed twice with PBS