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Ruman (Diagen) in 1993 using a solid phase synthesis 12 , a method which is based on the classic Merrifield synthesis 13 , where peptide is synthesized stepwise on a modified solid polystyrene support (resin)

Experimental physiology, 101(6), 743-753

They also are sensitive to environmental conditions and can change chemical composition if stored at the wrong temperatures or shaken too vigorously, increasing the risk of an immune response or decreasing their effectiveness

The lipid residue in the LC-MS injection vial was redissolved with 300 L of isopropanol-methanol (V:V=1:1), vortexed for 30 s, and sonicated with ice water for 3 min, then the solution was transferred to a 1.5 mL centrifuge tube, and left to stand at -20C for 2 h

This type of covalent binding inactivates important functional proteins, leading to hepatic cell death and enzymes like alanine aminotransferase (ALT) release into plasma 6