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The t of 46.3 h for the HBSS buffer (pH 7.4) also indicated the viable timeframes of the subsequent experiments that require physiological conditions, such as cell culture studies

10.3389/fneur.2015.00048 55 EngebretsenK

They activate some overlapping populations of neurons, but separate neurons, which, I think, is the basis for their additive effect

This means that if you take more than your body needs, the excess is generally excreted in your urine, which is why B12 is considered very safe, even at high doses