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Materials and methods Cell culture and characterization Human iPSCs (gifted from Bonyakhteh Stem Cell Research Center, Tehran, Iran) were maintained on mitomycin C-arrested mouse embryonic fibroblasts in an undifferentiated state 21 in DMEM/F12 (Gibco by Life Technologies, Paisley, Scotland, UK) medium supplemented with 1% non-essential amino acids, 20% knockout serum replacement, 4 ng/ml bFGF (all from Sigma-Aldrich Chemie GmbH, Steinheim, Germany), and 1 mM L-glutamine (Sigma-Aldrich, St

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