Western blot analysis Cells were washed with PBS containing 1 mmol L 1 N-Ethylmaleimide (NEM, Santa Cruz, #sc-202719), then lysed with Laemmli Sample buffer containing 2 % SDS, 4 % glycerol, 40 mmol L 1 Tris-Cl (pH 6.8), 5 % 2-Mercaptoethanol, 0.01 % bromophenol blue, 1 mmol L 1 NEM, 1 mmol L 1 Phenylmethylsulfonyl fluoride (PMSF), protease inhibitor mixture (Roche, #COEDTAF-RO), and phosphatase inhibitor mixture (Roche, #04906837001)
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Quek YLK, Li J, Fujimoto J, Zhang J, Chow CWB, Behrens C, Mao X, Correa A, Song X, Zhang J, Roarty EB, Thornton RL, Coyle MD, Antonoff MB, Kalhor N, Moran C, Weissferdt A, William Jr WN, Swisher SG , Lee JJ, Heymach JV, Wistuba IV, Futreal A, Zhang J
For injury repair research, is BPC-157 or TB-500 a better stack with IGF-1 LR3
Molecules , 19(11), 19066-19077