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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

For short term storage, peptides should be kept in their lyophilized (dry powder) form at 4C in a refrigerator

(10) Pinealon Peptide and Cellular Anti-aging Pinealon peptide is suggested to possess properties observed by researchers to mitigate cellular aging and longevity-related issues within the central nervous system

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The rate-limiting step of glutathione synthesis is cystine import through the cystine/glutamate transporter (Figure 1E), and its expression is induced under oxidative stress and during cell proliferation