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Samples and standards were diluted fourfold in sample dilution and plates were processed according to the manufacturers instructions

Typical daily range (educational / research reference): 12 mg once daily with gradual progression

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Because MOTS-c enhances insulin-independent glucose uptake, patients with type 1 diabetes or those using exogenous insulin should be monitored carefully to prevent hypoglycemia, though this theoretical risk has not materialized in clinical reports to date