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The eluate was further purified using SEC (Superose 6 increase 100/30 Cytiva) with the SEC buffer (20 mM Hepes pH7.5 and 400 mM NaCl)

Currently, he has severe persisting neuropathy despite intramuscular injection of hydroxocobalamin on alternating days, and the use of pregabalin, amitriptyline and a morphine preparation

Anthropometric assessment Participants body measurements were taken using standardized methods and equipment