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Cells were incubated with or without 250 mol l 1 succinylacetone (SA) for 48 h, and the cells were then lysed with NP40 lysis buffer (20 mmol l 1 Tris-HCl (pH 7.5), 150 mmol l 1 NaCl, 1% NP40)

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Each application becomes a calming ritual, leaving the face looking rested and remarkably smooth

Cell culture Erythroid progenitors from SCD patients were generated with peripheral blood mononuclear cells (PBMN) followed by CD34+ cell selection (#17856, StemCell Technologies)