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Strength and limitation Important factors limiting the analysis of results and its discussion in this study are the relatively small group of participants and significant differences between the protocol used in our study and those found in the literature

Twenty grams were blended for 2 minutes with cold chloroform-methanol mixture (2:1 v v -1 ) in proportion of 10 mL of solvent mixture per gram of tissue (except 20 ml of solvent per gram of liver tissue) in a Warring blender

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Gallagher S, Marras WS, Litsky AS, Burr D