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Prolonged fasting has been shown to suppress luteinizing hormone (LH) secretion, thereby reducing testosterone production as the body prioritizes energy conservation over reproductive processes [56, 57]

Brain Imaging Behav (2020) 14(2):56272

The stirring process continued for another 12 h at 50 C, after which the mixture was introduced into 10 mL of deionized water while being sonicated

These free radicals damage the brain cells and can increase brain cell death