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EJM and TX-MM-030h were maintained in Iscoves modified Dulbecos medium, supplemented with 20% fetal bovine serum (FBS) and insulin, selenium, transferrin (BD Biosciences) universal culture supplement (1:1000)

B12 is an essential B vitamin found in fish, meat, eggs, shellfish, and dairy

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(3) Plasma GPx (GPx3) is present in the mitochondria of several organs, such as the kidney, lung, epididymis, breast, heart, and muscle