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HIF signaling changes the extracellular matrix, regulates the tumor immune response, increases angiogenesis, and affects the tumor microenvironment, altering the metabolism of tumor and stromal cells and increasing tumor invasiveness and metastasis (131)

Both conditions (treatment and control) were analyzed in 3 biological triplicates for each cell line

Huang L, Yu YY, Kirschke CP, Gertz ER, Lloyd KK

This refluxing step was repeated twice, followed by centrifugation, and washing with ethanol