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All cultures were maintained in the presence of 1% penicillin- streptomycin

DOI 10.1111/jocd.16076

To minimize such unwanted events, we developed a standardized thawing procedure for frozen samples, with a small sample volume (150-200 L): thawing under strict cooling conditions (+0C to +2C) within 12-15 minutes in an ice-water bath, without agitation

Considering the T m values and binding affinities of all PV2 and PV3 mutants, PV2-S26pAcF, PV2-S71PAcF, PV3-S85pAcF and PV3-N77pAcF which showed significantly improved stability and binding affinity, were selected for 2 times 2 combination, resulting in four pairs of BsNbs

This gives it anxiolytic properties with a significantly lower risk of dependence or sedation