To investigate the functional role of the R6 region in the SIRT1 promoter, a series of luciferase reporter constructs was obtained from VectorBuilder (USA), including a promoterless basic vector, a construct containing the full-length human SIRT1 promoter, including the R6 site, and promoter fragments corresponding to regions upstream and downstream of the R6 site that lack the R6 region (vector IDs: VB260105-1480rwj, VB260105-1503wsw, VB260105-1505cps, and VB260105-1513uay)
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Uricases are employed in the treatment of refractory gout and are capable of achieving a rapid reduction in hyperuricemia, significant resolution of tophi, alleviation of chronic joint pain, and enhancement of overall quality of life
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It is found in nearly every cell in the human body, acting as a primary line of defense against oxidative damage