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The biopsies were treated with metformin (10 mM) or H 2 O control in complete M199 medium (1 mL volume)

Thigh injections are more painful than abdominal, but injection volumes up to 0.8 ml typically do not increase pain in either site

"Not FDA approved" does not automatically mean illegal or worthless

Neurodegenerative diseases such as Alzheimers disease (AD), Parkinsons disease, amyotrophic lateral sclerosis (ALS), and Huntingtons disease represent an escalating global health challenge, driven by an aging population and the lack of effective disease-modifying therapies

After glucose depletion in YP20D medium, a feeding solution containing 200 g/L yeast extract, 100 g/L peptone, and 600 g/L glucose was continuously supplied