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Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development

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As a naturally occurring chemical, GHRH derives from the hypothalamus, an area of the brain that coordinates the bodily system (i.e., the endocrine system) responsible for controlling just about every important function needed for internal stability growth, energy, metabolism, and reproduction, as well as responses to injury, stress, and mood.4 5 True to its name, GHRH causes the pituitary gland to release growth hormone

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