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Supporting a dominant role for diet composition in establishing the Firmicutes : Bacteroidetes ratio, previous work has shown that mice maintained on a high-fat diet had a similar cecal Firmicutes : Bacteroidetes ratio to those maintained on a high-carbohydrate diet 77

10.3389/fmicb.2023.1118529 229 KatrinliS.OliveiraN

Others feel the greatest benefit the following morning

Its copper-dependent superoxide dismutase activity contributes to cellular protection against oxidative damage, a primary driver of biological aging across tissue types

To normalize target gene expression levels, the relative expression of the gene in each sample versus a control in comparison to the -actin gene was calculated according to the 2-Ct method (41)