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The resulting PCR products were purified using AxyPrep Mag PCR Clean-Up Kit (Axygen, United States), validated by an Agilent 2,100 Bioanalyzer (Agilent Technologies, United States), and quantified using a Qubit 2.0 Fluorometer (Invitrogen, United States)

Brown Univ Child Adolesc Psychopharmacol Updat 22:67

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however, drinking even one cup of espresso at the higher end of the scale will exceed the recommended limit of 200 mg/day (Coppi et al., 2023

Mesenchymal Stem Cells (MSCs) to downregulate hyperarousal pathways and suppress neuroinflammatory cascades associated with chronic insomnia and related psychiatric comorbidities