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For quantitative analyses, ratio of cells positive for nuclear -catenin staining to total number of cells was counted as pixel intensity ratio positive for nuclear localization using Image J (NIH, Bethesda, MD, USA)

Weight loss starts slowly in the first 4 weeks of treatment, with changes in appetite and a feeling of fullness with fewer cravings

Current strategies to enhance adipose stem cell function: an update

Furthermore, being complementary to CD, Raman spectroscopy is immune to the effects of light scattering, and can therefore be used for the analysis of proteins in micelles and other scattering media (Chi et al., 1998)

Never use saline solution