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Avoid repeated freeze-thaw cycles after reconstitution, as this degrades peptide integrity

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Helps with detoxification

Moreover, in many cases, the administration of these drugs over a long period is not very effective, and it often leads to the recurrence of the disease (Melo et al

Western blot analysis One microgram of SYN was added to denaturing sample buffer (62.5 mM Tris, pH 6.8, 10 % (v/v) glycerol, 2 % (w/v) SDS, 5 % (v/v) -mercaptoethanol, and 1 % (w/v) bromophenol blue), boiled for 90 s and subjected to polyacrylamide gradient (416 %) gel electrophoresis under denaturing conditions followed by transfer to polyvinylidene difluoride (PVDF) membranes (PerkinElmer, Waltham, MA)