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On DIV7 the cells were treated with vehicle (HDIV 12 the neurons were fixed (4% paraformaldehyde, 3% sucrose, 60 mM PIPES, 25 mM HEPES, 5 mM EGTA, 1 mM MgCl 2 0) or peptides (as described in the text) added to media

Its described as stable, which means it holds up well, even in the presence of stomach acid

That sounds like a failure

doi:10.7860/JCDR/2016/18208.8127 If a good first response is seen, a second injection may be considered when the improvement from the first injection begins to taper off

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