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To model the breakdown of the animals main energy reserve, we measured how triglycerides (TAGs) stored in intestinal lipid droplets 17 were altered after 24 h of fasting compared to a previously reported 48-h fast 18

Oxidatively fragmented phospholipids as substrates

These frameworks could also support the integration of experimental outputs with computational models and digital twin platforms, enabling advanced data analytics, cross-laboratory reproducibility, and multi-scale modeling of physiological processes

We then performed untargeted metabolomics and found 1154 nonredundant metabolites in the BM microenvironment (Fig