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The sections were then incubated with primary antibodies against occludin (Abcam, dilution 1:100) and ZO-1 (ThermoFisher, dilution 1:100), diluted in PBS containing 0.2% BSA and 0.1% saponin at 4 C overnight, washed, and incubated for 1 h with Alexa Fluor 647 donkey anti-rabbit IgG secondary antibody (Invitrogen, dilution 1:400)

The effect of combined drug treatment on rheologic properties of the blood in patients with disordered circulatory encephalopathy

Peptide therapy is not recommended for individuals who are: -Pregnant or breastfeeding -Diagnosed with active cancer or hormone-sensitive tumors or high risk -Undergoing immunosuppressive therapy (unless cleared by their specialist) -Experiencing uncontrolled endocrine disorders Always consult your provider and disclose your full medical history before starting any peptide protocol

Journal of Circulation , 1995, 92:2825-30

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